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KMID : 0616120080390020110
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Korean Journal of Pharmacognosy
2008 Volume.39 No. 2 p.110 ~ p.117
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Inhibition of LPS induced iNOS, COX-2 and cytokines expression by salidroside through the NF-κB inactivation in RAW 264.7 cells
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Won So-Jung
Lee Kyung-Tae
Park Hee-Juhn
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Abstract
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In this study, we investigated the anti-inflammatory effects of salidroside (SAL) isolated from the MeOH extract of Acer tegmentosum Maxim heartwood in RAW 264.7 macrophage cells. SAL pretreatment significantly inhibited nitric oxide (NO) and prostaglandin E2 (PGE2) productions in the lipopolysaccharide (LPS)-induced RAW 264.7 cells. Western blot and RT-PCR analyses revealed that SAL inhibited the LPS-induced expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the protein and mRNA levels in a concentration-dependent manner. In addition, SAL reduced the release and the mRNA expressions of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Furthermore, nuclear factorkappa B (NF-κB) luciferase reporter assay was performed to know the involvement of SAL in the production of pro-inflammatory cytokines, we confirmed that LPS-induced transcription activity of NF-κB was inhibited by SAL. Taken together, our data indicate that anti-inflammatory property of salidroside might be the result from the inhibition of iNOS, COX-2, TNF-α and IL-6 expressions via the down-regulation of NF-κB activity.
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KEYWORD
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salidroside, Acer tegmentosum Maxim, LPS, NF-κB, Anti-inflammation
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